The ELISA (enzyme-linked immunosorbent assay) is a widely used application by researchers to detect and quantify from various samples of proteins and antigens. Thanks to the continual scientific and research work that has made available different target-specific ELISA kits from various manufacturers from different parts of the world. These cytokine ELISA kits products such as CCL2 ELISA are used for streamlining a researcher’s immunodetection experiments across different laboratories. 

What are cytokines?

These are small signaling proteins defined as soluble molecules, being a product of a cell to act on another, which starts a response to immune response, infection, cancer, apoptosis, and development. However, signaling happens due to binding to certain surface receptors that initiate intracellular signal cascades and drive the regulation and control of genes. The cytokines have family members which are made up of the following 

• Interleukins
• Interferons
• Chemokines 
• Tumor necrosis factors
• Bone morphogenic proteins

https://microbenotes.com/cytokines-mechanism-of-action-and-functions/

They are distinct from hormones in that multiple cell types may produce cytokines. Moreover, at a much lower concentration, they have a higher level of effectiveness. Thankfully, there are many prominent IL-12 elisa kits designed and manufactured to detect and quantify specific cytokines. As a research tool, this ELISA kit and others make research work convenient, hence why they are an important and useful tool in biomedical research. 

Cytokines ELISA kit Products

Designed to facilitate and make research work easier, the following are the cytokines Elisa kits products you can choose for your research work.

1. Human IL-6 Quantikine ELISA Kit

This is an immunoassay 4.5-hour solid-phase designed to measure IL-6 in cell culture supernates, serum, and plasma. Human IL-6 Quantikine ELISA Kit has recombinant human IL-6 and antibodies that have quantified the recombinant factor’s accuracy. According to the results from this ELISA kit product using natural IL-6 revealed linear curves parallel to the standard curves obtained with the use of E. coli-expressed Quantikine kit standards. The result means Human IL-6 Quantikine ELISA kit is perfect for determining a mass value for natural Human IL-6 Quantikine.                                         

 2. Rat TNF-alpha Quantikine ELISA Kit

This Quantikine Rat TNF-alpha Immunoassay is a 4.5-hour solid-phase ELISA designed to measure rat TNF-alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat TNF-alpha and antibodies raised against the recombinant factor. However, the kit has proved to quantify the recombinant rat TNF-alpha accurately. The results shown from using this kit revealed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. This indicates that this Rat TNF-alpha Quantikine ELISA Kit is accurate for the determination of mass values for natural rat TNF-alpha.

3. Human IGF-I/IGF-1 Quantikine ELISA Kit

This Immunoassay is a 4.5-hour solid-phase ELISA designed to measure human IGF-1 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IGF-1 and has antibodies raised against the recombinant protein. The results shown from using this kit revealed naturally occurring human IGF-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. With the results from this kit, researchers can use it to determine mass values for natural human IGF-1.

4. Human IL-2 Quantikine ELISA Kit

The Quantikine Human IL-2 Immunoassay is a 4.5-hour solid-phase ELISA designed to measure IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-2 and antibodies raised against recombinant human IL-2. Human IL-2 Quantikine ELISA kit has proved to quantitate the recombinant factor perfectly. Results obtained using natural human IL-2 showed dose-response curves parallel to the standard curves obtained using the recombinant Quantikine kit standards. With the results from this kit, researchers can use it to determine mass values for natural human IL-2. 

5. Mouse IL-6 Quantikine ELISA Kit

The Quantikine Mouse IL-6 Immunoassay is a 4.5-hour solid-phase ELISA designed to measure mouse IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-6 and antibodies against the recombinant factor. This Immunoassay has been shown to quantitate the recombinant mouse IL-6 accurately. Results obtained using natural mouse IL-6 showed dose-response curves parallel to the standard curves obtained using the Quantikine mouse kit standards. These results mean researchers can employ this kit in determining relative mass values for natural mouse IL-6.

6. Mouse IL-4 Quantikine ELISA Kit

The Quantikine Mouse IL-4 Immunoassay is a 4.5-hour solid-phase ELISA designed to measure mouse IL-4 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-4 and antibodies raised against the recombinant factor. This Immunoassay has been shown to quantitate the recombinant mouse IL-4 accurately. Results obtained using natural mouse IL-4 showed dose curves parallel to the standard curves obtained using the recombinant Quantikine kit standards. This indicates that this IL4 ELISA Kit is accurate for the determination of mass values for natural mouse IL-4.

Conclusion

With different kinds of cytokine Elisa kits products, researchers are provided with wider options to choose from, depending on what they are researching. It is, however, important to choose the right kit for the right research for an effective result. ELISAs are a type of immunoassay that use antigens and antibodies to detect the presence of specific proteins in a sample. This process is often used in medical fields to help detect diseases or toxins.

ELISA kits test for cytokines, which are molecules that regulate physiological functions such as inflammation and immune response.ELISA kits are used to detect specific antibodies in the blood. ELISA is a very cheap and quick test to detect the presence of different drugs or markers like HIV, hepatitis, and cancer markers. ELISA kits are commonly used when blood isn’t available for testing.

ELISA stands for Enzyme-Linked Immunosorbent Assay. It is a laboratory technique that detects the presence of specific antibodies in biological samples, like blood, serum, or urine. An ELISA kit is a type of assay that can be used to identify cytokine levels. The ELISA kits are designed to detect and measure the levels of six different proteins in one sample at once. ELISA kits are used to detect the presence of a certain antigen in blood serum or body fluids. This is typically done using antibodies and bioreagents, such as antigens, in order to test the patient’s immune system. With this kit, researchers can use it to determine mass values for natural human IL-2.